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Recombinant Enzyme Carboxypeptidase B for Mass Spectrometry with 170u / mg pro.

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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Tel: 86-21-54336592
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Recombinant Enzyme Carboxypeptidase B for Mass Spectrometry with 170u / mg pro.

Brand Name : YaxinBio
Model Number : RCPB01-01
Certification : ISO 9001
Place of Origin : China
MOQ : 10mg
Payment Terms : T/T
Packaging Details : ice packaging
Price : $175/10mg
Supply Ability : 1000g per week
Delivery Time : 6 days
Purified by : HPLC
CAS : 9025-24-5
EC : 3.4.17.2
Cat. No. : RCPB01-01
Specific activity : ≥170 u/mg pro
Purity : > 95%
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Recombinant Enzyme Carboxypeptidase B for Mass Spectrometry with 170u / mg pro


Description


Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 33.8kDa is purified by

standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign

enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free

from protease inhibitors such as PMSF and EDTA.


Main Features


Source: Escherichia Coli.

Physical Appearance: Sterile Filtered lyophilized powder.

Formulation: The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.

Solubility: It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile

18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


Unit Definition

One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.


Advantages


Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of

contamination and decreases the chances of activity loss in the process of transport and storage.

High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates

contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.

Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3,

eliminate the risk of virus presence, or of any other potential adventitious agents found in

animal-derived carboxypeptitase B


Recommend Usage


To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The

ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in

sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃,

It is stable within 24 months and above 90% activity remained after 10 times repeated freezing

and thawing.


Product Tags:

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