Recombinant Enzyme Carboxypeptidase B for Mass Spectrometry with
170u / mg pro
Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a
Mw of 33.8kDa is purified by
standard chromatography techniques. Recombinant Rat
Carboxypeptidase-B is free from foreign
enzymes such as carboxypeptidase A & chymotrypsin. Recombinant
Carboxypeptidase-B is free
from protease inhibitors such as PMSF and EDTA.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered lyophilized powder.
Formulation: The protein was lyophilized with 100mM NaCl and 20mM
Solubility: It is recommended to reconstitute the lyophilized Rat
Carboxypeptidase-B in sterile
18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to
other aqueous solutions.
One Unit of carboxypeptidase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25℃, pH 7.65.
Stability:A sterile recombinant carboxypeptidase B lyophilized
eliminates the risk of
contamination and decreases the chances of activity loss in the
process of transport and storage.
1) Recombinant carboxypeptidase B provides increased specific
activity and eliminates
contaminating proteases activities found in extracted enzymes with
lower purity level.
2) No other contaminating proteases such as chymotrypsin and
3)Less than 10ppm of recombinant trypsin.
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs
to the AOF level 3,
eliminate the risk of virus presence, or of any other potential
adventitious agents found in
animal-derived carboxypeptitase B
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The
ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2℃-8℃ in
sealed container. It is stable within 24 months.After dissolved, it
should be stored under -20℃,
It is stable within 24 months and above 90% activity remained after
10 times repeated freezing