Rcombinant Carboxypeptidase B Lyophilized Powder Animal Origin Free
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase)
catalyzes the hydrolysis of the basic amino acids, lysine,
arginine, and ornithine from the C-terminal position of
polypeptides. It has been shown to be a single polypeptide of 33.8kD
. Trypsin is capable of converting native enzyme to the active
enzyme, carboxypeptidase B II in vitro. The optimum pH is found to
be 9.0. The enzyme may be used for sequence analysis by successive
cleavage of C-terminal basic amino acids. It can also be used as a
serum marker for the diagnosis of acute pancreatitis.
Form:Lyophilized from 20 mM Tris, pH 8.0 + 50 mM NaCl.
Enzyme Commission Number: 22.214.171.124
White or white-like or yellowish lyophilized
Tris salts, NaCl salts and carbohydrates
35% ～ 60%
Single main band on SDS-PAGE
Less than 10 ppm of recombinant trypsin.
One Unit of carboxypeptidase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25℃, pH 7.65.
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2℃-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
stable within 24 months and above 90% activity remained after 10
times repeated freezing and thawing.