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Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd.
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Rcombinant Carboxypeptidase B Lyophilized Powder Animal Origin Free

Shanghai Yaxin Biotechnology Co.,Ltd.
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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Tel: 86-21-54336592
Contact Person:
Mr zhao

Rcombinant Carboxypeptidase B Lyophilized Powder Animal Origin Free

Brand Name : YaxinBio
Model Number : RCPB01
Certification : ISO 9001
Place of Origin : China
MOQ : 10mg
Payment Terms : T/T
Packaging Details : ice packaging
Price : 1750$/g
Supply Ability : 100000g per year
Delivery Time : 7 work days
Product : Recombinant Carboxypeptidase B
Cat. No : RCPB01
Specific activity : ≥170 u/mg pro
Packaging : 10mg,100mg,1g,bulk
Manufacture : YaxinBio
Contaminant activity : No chymotrypsin,carboxypeptidase A,and other protease contaminant.Less than 10ppm of recombinant trypsin.
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Rcombinant Carboxypeptidase B Lyophilized Powder Animal Origin Free


Description
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 33.8kD. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Source:E. coli
Species:Rat
Form:Lyophilized from 20 mM Tris, pH 8.0 + 50 mM NaCl.
Enzyme Commission Number: 3.4.17.2

Main Features


Source

E. Coli

Purified by

HPLC

Format

White or white-like or yellowish lyophilized

Additives

Tris salts, NaCl salts and carbohydrates

Protein content

35% ~ 60%

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.


Unit Definition


One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.


Recommend Usage


To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.


Email:Zhaozhi@yaxinbio.com



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