Stable Recombinant Carboxypeptidase B with E.coli Carboxypeptidase B E.coli
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase)
catalyzes the hydrolysis of the basic amino acids, lysine,
arginine, and ornithine from the C-terminal position of
polypeptides. It has been shown to be a single polypeptide of 33.8
kDa. Trypsin is capable of converting native enzyme to the active
enzyme, carboxypeptidase B II in vitro. The optimum pH is found to
be 9.0. The enzyme may be used for sequence analysis by successive
cleavage of C-terminal basic amino acids. It can also be used as a
serum marker for the diagnosis of acute pancreatitis.
Synonyms:carboxypeptidase B; protaminase; CPB1; pancreatic
carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine
[L-arginine]hydrolase; EC 22.214.171.124; 9025-24-5
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs
to the AOF level 3, eliminate the risk of virus presence, or any
other potential adventitious agents found in animal-derived
Stability:A sterile recombinant carboxypeptidase B lyophilized
eliminates the risk of contamination and decreases the chances of
activity loss in the process of transport and storage.
1) Recombinant carboxypeptidase B provides increased specific
activity and eliminates contaminating proteases activities found in
extracted enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and
3)Less than 10ppm of recombinant trypsin.
White or white-like or yellowish lyophilized
Tris salts, NaCl salts and carbohydrates
35% ～ 60%
≥170 units/mg pro.
Single main band on SDS-PAGE
Less than 10 ppm of recombinant trypsin.
One Unit of carboxypeptidase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25℃, pH 7.65.
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2℃-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
stable within 24 months and above 90% activity remained after 10
times repeated freezing and thawing.